Fig. 1.

Representative western blot of gills taken from Atlantic killifish acclimated for ≥14 days to freshwater (FW), seawater (SW) or 2× SW. Gills were rapidly excised from fish, briefly rinsed in teleost Ringer solution, and incubated without (A) or with (B) 10 μmol l⁻¹ forskolin for 15 min at room temperature. Each lane represents gill samples pooled from 6 individual fish. Equal amounts (30 μg) of SDS-solubilized protein were loaded in each lane and probed with anti-phospho Na⁺–K⁺–Cl⁻ cotransporter antibody R5. The R5 antibody recognized a strongly stained band centered at ~170 kDa.