Fig. 2.
Time course of NKCC1 cotransporter phosphorylation in excised killifish gills stimulated with forskolin. Gills (three pieces per fish) were excised from fish maintained in SW and incubated in teleost Ringer solution containing 10 μmol l−1 forskolin at room temperature. p-NKCC was analyzed on dot blots using the anti-p-NKCC antibody R5 and normalized for total protein. Data are means ± s.e.m. of 2–6 fish at a given time point. One-way ANOVA determined a significant effect (P<0.001) of time on p-NKCC. Asterisks indicate a significant difference (P<0.05) from time zero.