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. 2010 Apr 16;213(9):1558–1566. doi: 10.1242/jeb.039644

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Fig. 6. — Time course of total NKCC1 protein expression (A) and NKCC1 phosphorylation (B) after transition of fish from FW to SW. Each point represents the mean ± s.e.m. of 6 individual fish in a cohort of fish studied in one experiment. Different symbols represent different experiments. One-way ANOVA determined a significant effect of time on total NKCC1 in all three experiments (P<0.002), and a significant effect of time on p-NKCC in one experiment (P=0.023; open circles). Asterisks indicate a significant difference (P<0.05) from pre-treatment control within an experiment.