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Time course of total NKCC1 protein expression (A) and NKCC1 phosphorylation (B) after transition of fish from SW to 2× SW. Each point represents the mean ± s.e.m. of 6 individual fish in a cohort of fish studied in one experiment. Different symbols represent different experiments. One-way ANOVA determined a significant effect of time on both total NKCC1 (P=0.003) and p-NKCC (P=0.004). Asterisks indicate a significant difference (P<0.05) from pre-treatment control within an experiment.
