Figure 4.

Effects of TNFalpha and tamoxifen on ERalpha expression in MCF-7 and MCF-7CLU cells. Total cell lysates prepared from MCF-7 (Panel A) and MCF-7CLU (Panel B) cells treated with ethanol vehicle, TNFalpha (1, 2.5 and 5 ng/ml) or tamoxifen (1, 2.5 micromolar) in serum free medium for 48 h were separated on 10% SDS-PAGE gels and immunoblotted with mouse monoclonal antibodies against ERalpha and GAPDH. Individual band intensity was normalized against GAPDH and plotted relative to appropriate control. Blots are representative of three independent experiments. Results are expressed as Mean ± SD, *, p < 0.05 (control versus treated).