Figure 2A.

Different staining of femoral artery. Hematoxylin and eosin (H and E) staining was used to determine the general morphology of the blood vessels; the pinkish part represents the cytoplasm and the blue spots show the presence of nucleus in the artery wall. Masson trichrome staining was used for determination of collagen in tissue; the blue staining at the tunica adventitia represents the presence of collagen in the blood vessel; van Gieson staining was used to determine the presence of elastin in tissues; the blackish-brown staining in the tunica intima shows the presence of elastin in the artery.