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. Author manuscript; available in PMC: 2010 Apr 19.
Published in final edited form as: J Neuropathol Exp Neurol. 2008 Oct;67(10):954–962. doi: 10.1097/NEN.0b013e3181875b2f

FIGURE 3.

FIGURE 3

Graft detection of implanted canine olfactory bulb-derived neural progenitor cells containing superparamagnetic iron oxide particles (MPIOs). (A–D) Dog 2. (A) In vivo and (B) ex vivo magnetic resonance images show the MPIO-labeled implant (arrowhead) at 8 days and 4 weeks posttransplantation, respectively. At 4 weeks postimplantation, the graft is detected by histology in the region corresponding to the magnetic resonance image by the presence of brown-gold MPIOs (C) and by fluorescent microscopy (D). (E–G) Dogs 7 and 8. (E, F) Representative ex vivo magnetic resonance images of coronal sections 8 weeks postimplantation from 2 dogs. The graft tracts (arrows) are visible descending through the parietal cortex in Dog 7 (E) and through the (F) corona radiata/internal capsule in Dog 8 (F). (G) Hematoxylin and eosin-stained section (horizontal plane) of the graft tract showing an aggregate of MPIO-containing cells. The MPIOs are clearly visible as brown-gold spheres within cells. Scale bars = (C, D) 200 μm; (G) 25 μm.