FIGURE 4.
Dogs 5 and 6. Confocal Z-stack images of superparamagnetic iron oxide particle (MPIO)-labeled canine olfactory bulb-derived neural progenitor cell immunostaining. Cells in the cortical graft region stain positively for nestin (A, B) and for glial fibrillary acidic protein (GFAP) (C, D). The arrows indicate non-MPIO-labeled nestin+ (B) and GFAP+ (D) cells. (A1) Enlarged view of the box in (A) showing MPIOs in a nestin+ cell. (C1) Enlarged view of the box in C indicating a GFAP+ cell containing MPIOs (arrowhead). The step-size of the sequences was 0.04 μm. Scale bars = (A–D) 20 μm; (A1, C1) 10 μm.
