FIGURE 5.
β-Glucuronidase gene expression. (A–D) Dog 1. (A) In vivo magnetic resonance imaging, sagittal section, of a graft (arrowhead) colabeled with superparamagnetic iron oxide particles (MPIOs) at 8 days posttransplantation. (B) Enzyme histochemistry of MPIO-labeled canine olfactory bulb-derived neural progenitor cells shows red β-glucuronidase staining (arrows) at 8 days postimplantation. (C, D) Higher magnification views show that β-glucuronidase+ cells contain MPIOs. (E–G) Dog 3. β-Glucuronidase cells are detected in the (E) tela choroidea and in the (G) ependyma at 4 weeks posttransplantation (sagittal sections). These cells were not labeled with MPIOs. (F) Higher magnification view of the region delineated by the box in (E). Scale bars = (B) 50 μm; (C, D, F, G) 20 μm; (E) 200 μm.
