Table 2.
Spontaneous reversion rates of yeast strains with pms1 mutations
| Lys+ Reversion Rate (× 10−6) |
His+ Reversion Rate (× 10−8) |
Conservation among 38 organisms* |
||||
|---|---|---|---|---|---|---|
|
Pms1 Genotype |
Rate (95% CI) |
Relative Rate |
Rate (95% CI) |
Relative Rate |
Pms1 homologs |
Mlh1 homologs |
| WT | 0.093 (0.064–0.13) | 1 | 0.7 (0.5–0.9) | 1 | - | - |
| Δ | 1100 (840–2200) | 12,000 | 43 (33–62) | 61 | - | - |
| R170E/K172E | 530 (430–650) | 5,700 | 22 (21–30) | 31 | 21/26 | 0/0 |
| K190E | 0.15 (0.12–0.22) | 2 | 0.7 (0.4–0.9) | 1 | 38 | 35 |
| K194E | 0.58 (0.39–0.73) | 6 | 1.4 (0.9–1.7) | 2 | 37 | 35 |
| K197E | 83 (59–110) | 890 | 4.1 (2.3–4.8) | 6 | 38 | 0 |
| R198E | 27 (23–31) | 290 | 4.6 (3.1–5.7) | 7 | 38 | 0 |
| K218E | 270 (220–410) | 2900 | 12 (11–20) | 17 | 38 | 0 |
| K229E | 1.1 (0.82–2.0) | 12 | 0.9 (0.6–2.3) | 1 | 29 | 38 |
| R243E/K244E | 93 (75–130) | 1,000 | 5.9 (4.3–7.0) | 8 | 30/6 | 1/0 |
| R256E | 0.22 (0.18–0.34) | 2 | 0.9 (0.7–1.2) | 1 | 5 | 18 |
| R306E | 4.1 (2.0–6.5) | 44 | 1.5 (0.9–2.1) | 2 | 38 | 0 |
| R311E | 5.9 (5.0–13) | 63 | 2.1 (1.4–3.1) | 3 | 37 | 0 |
| K328E** | 11 (8.3–19) | 60 | 3.4 (3.0–7.9) | 3 | 37 | 37 |
Organisms and NCBI reference sequence numbers for Pms1 and Mlh1 homolgs:
Saccharomyces cerevisiae (NP_014317, NP_013890), Vanderwaltozyma polysporan (XP_001644784, XP_001645690), Candida glabrata (XP_447944, XP_446304), Ashbya gossypii (NP_985276, NP_985351), Kluyveromyces lactis (XP_452705, XP_453504), Penicillium marneffei (XP_002145249, XP_002144802), Podospora anserine (XP_001910360, XP_001910200), Aspergillus fumigatus (XP_755674, XP_753480), Aspergillus clavatus (XP_0001275615, XP_001274465), Coccidioides immitis (XP_0012145606, XP_0012239552), Neosartorya fischeri (XP_001260801, XP_001259525), Aspergillus terreus (XP_001208682, XP_001211278), Magnaporthe grisea (XP_363790, XP_367196), Gibberella zeae (XP_382105, XP_389904), Aspergillus oryzae (XP_001821792, XP_001824017), Tribolium castaneum (XP_974934, XP_972898), Sclerotinia sclerotiorum (XP_001589152, XP_001590867), Aspergillus niger (XP_001399369, XP_001398763), Strongylocentrotus purpuratus (XP_786592, XP_793318), Bos Taurus (XP_605426, XP_001790604), Populus trichocarpa (XP_002321013, XP_002325965), Mus musculus (NP_032912, NP_002400), Aspergillus nidulans (XP_663920, XP_657730), Canis familiaris (XP_536879, XP_534219), Homo sapiens (NP_000526, NP_000240), Danio rerio (XP_693648, NP_002165510, XP_002156705), Trichoplax adhaerens (XP_002110081, XP_002111226), Pan troglodytes (XP_001141671, XP_001170390), Equus caballus (XP_001494013, XP_001489268), Acyrthosiphon pisum (XP_001947410, XP_001945011), Oryza sativa (NP_001047292, NP_001045457), Monodelphis domestica (XP_001377577, XP_001362893), Arabidopsis thaliana (NP_567236, NP_567345), Anopheles gambiae (XP_308635, XP_320342), Schizosaccharomyces pombe (NP_594417,NP_596199) and Rattus novergicus (NP_001099378, NP_112315).
From [38].
Mutation rates were measured as described in Materials and Methods. Rates are the median for 12 independent cultures.