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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: J Immunol. 2010 Feb 8;184(6):2793–2804. doi: 10.4049/jimmunol.0902846

Figure 4. Small but not large pre-selection DP thymocytes are acutely sensitive to reduced intracellular Bcl-xL.

Figure 4

Total Mybf/w Cd4-Cre Tcrα−/− and Mybf/f Cd4-Cre Tcrα−/− thymocytes were depleted of DP thymocytes using MACS CD4 MicroBeads. Negatively selected cells were subsequently cultured for 20 or 36 hrs and analyzed by flow cytometry. Results are representative of three separate experiments. (A) Histogram overlays present the change in FSC of Mybf/w Cd4-Cre Tcrα−/− and Mybf/f Cd4-Cre Tcrα−/− DP thymocytes from 20 to 36 hrs post culture. (B) Intracellular staining for Bcl-xL measured by flow cytometry at 20hrs and 36 hrs post culture through a live DP thymocyte gate. (C) Assessment of DP thymocyte survival after 20 hrs and 36 hrs in culture by staining for CD4, CD8, 7AAD and Annexin 5. Results are presented as mean +/− SEM. n≥3, *p < 0.005 (Student’s t-test).