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Western blot analysis of dysferlin and myogenic differentiation proteins with GAPDH as a loading control during each day of the differentiation time course (Days 1 – 7) in parental C2C12 P9 cells and dysferlin-deficient C2C12 cells treated with vehicle control (-) or 100nM Dex (+). A. Parental C2C12 cells (P9). B. Dysferlin-deficient C2C12 cells (shRNA).
