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. 2010 Apr 19;5(4):e10060. doi: 10.1371/journal.pone.0010060

Figure 2. ICI 182780 but not MPP inhibits anchorage independent growth of GH3 cells.

Figure 2

GH3 cells were cultured on soft agar in complete medium containing ICI (10 nM) or (MPP 10 nM) as described in the materials and methods. A] Bright field microscopy shows GH3 cell colonies in soft agar. B] Quantitative analysis of GH3 cell colonies in soft agar following anti-estrogen treatment. Colonies were counted in at least 3 independent fields. Data is calculated as % of control and it is the mean ± SEM of 4 separate experiments. * indicates significant differences from control (p<0.05).