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A) Bright-field (scale bar, 500 µm) microscopic images showing undifferentiated colony morphology of hESC line 08/013 (p40) cultured in RegES medium and passaged with a single-cell dissociation method for 6 passages. B) Bright-field (scale bar, 500 µm) microscopic image showing undifferentiated colony morphology of hESC line 07/046 (p33) after freezing and subsequent thawing in RegES medium at passage 1.
