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Western blot against A) nebulin Z-repeats M176–M181 and B) serine rich domain, performed on neonatal day 5 samples (n=4) versus adult (4 mo. n=6). Both M176–181 and the serine rich domain are significantly upregulated in the adult. Shown are results normalized to the nebulin protein level, as obtained by the PonceauS-stained membrane (see Methods), with the average value for the neonates set to 100%. Example results are shown in insets. N = neonate, A = adult.
