Table 1.

Transcript analysis in soleus and TC muscle. Adult expression is shown relative to that of the neonate with exons that are up-regulated shown in green (and with positive values) and exons that are down-regulated in red (and with negative values). The protein modules encoded by the exons colored in correspondence to the regions of nebulin in Figure 1 bottom; their molecular weights are shown in kDa. Nomenclature adapted from (37) and(9). (Exon numbers in parenthesis are according to (26), see Methods for details.) The expected differences in the average molecular weight of adult nebulins is calculated based on the fold difference (See Methods for details). According to these results, the nebulin transcript is larger in the adult than in the neonate and is predicted to result in a nebulin protein that is 13.1 kDa larger in the soleus and 3.6 kDa in the TC.

SOLEUS
Exon #	Encodes	Mw (kDa)	Fold	Expected Contrbution to Mw (kDa) Ad vs neonate
8	M3/M4	4.08	2.199	2.23
11	M6/M7	4.11	2.063	2.12
13	M8/S1R1	4.41	−2.199	−2.40
14	S1 R1/R2	4.20	−2.301	−2.38
18	S1 R5/R6	4.08	−2.180	−2.21
19	S1 R6/R7	4.04	−2.324	−2.30
21	S2 R1/R2	4.86	−2.336	−2.78
25	S2 R5/R6	4.02	−2.166	−2.16
26	S2 R6/R7	4.04	−2.053	−2.07
33	S3 R7/ S4 R1/R2/R3	12.33	−2.136	−6.56
45	S6 R7/ S7/R1/R2/R3	12.12	2.191	6.59
133 (134)	S22 R3/R4	4.09	2.031	2.08
134 (135)	S22 R4/R5	3.92	2.098	2.05
135 (136)	S22 R5/R6	4.32	2.527	2.61
136 (137)	S22 R6/R7	4.33	2.101	2.27
137 (138)	S22 R7/M163	3.79	2.000	1.90
138 (139)	M163/M164	3.91	2.307	2.22
145 (146)	M170/M171	4.34	−2.336	−2.48
146 (147)	M171/M172	3.96	−2.213	−2.17
153 (154)	M177/M178b	3.58	2.428	2.10
154 (155)	M177/M178c	3.55	2.617	2.19
155 (156)	M177/M178d	3.48	2.084	1.81
163 (164)	M185/Ser	5.31	2.373	3.07
164 (165)	Ser	3.82	3.309	2.67
165 (166)	SH3	6.32	4.034	4.75
	Cummulative Difference:			13.1 kDa
TC
Exon #	Encodes	Mw (kDa)	Fold	Expected Contrbution to Mw (kDa) 5d/Ad
127 (128)	S21 R5/R6	4.16	7.465	4.72
	Cummulative Difference:			3.6 kDa