The structure of the LAT-associated ves1β gene is shown in schematic. Exons which are a part of the translated open reading frame are shown as thick white bars and introns as numbered, narrow gray bars. The narrow black line represents 5'- and 3'-untranslated sequences. The orange segments within the exons are patches of sequence conserved among ves genes (adapted from [30]). The segments incorporated into recombinant immunogens are indicated as follows: fragments included in the r-v1β(f1-3) immunogen are indicated by blue arrows labeled “F1”, “F2”, and “F3”, whereas sequences contained in the r-v1β(765) immunogen are indicated by the red arrow labeled “1–765”. The position of the peptide incorporated in the v1β(peptide) immunogen is indicated by a green bar. Note that only exon-encoded sequences taken from a mature mRNA-derived cDNA were included in each immunogen. “CKRD” indicates the position of the cysteine-lysine-rich domain. The black bar near the 3' end indicates the predicted transmembrane domain, and “ATG” and “TAG” are the translational start and stop codons, respectively.