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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Mol Biochem Parasitol. 2010 Mar 10;171(2):81–88. doi: 10.1016/j.molbiopara.2010.03.001

Figure 4. Confirmation of VESA1b identity through re-capture IP of VESA1 subunits.

Figure 4

B. bovis C9.1 line VESA1 was immunoprecipitated directly from non-denatured (N) or 8M urea-denatured (D) antigen. Alternatively, antigen was captured from non-denatured antigen with mAb 4D9.1G1, then was released by denaturation with 8M urea, diluted to allow antibody binding, and re-captured with a second antibody of different specificity. The order of reagents used in this experiment is indicated, as well as the nature of the antigens. The designation “U” refers to uncaptured antigens left over after recapture with the indicated antibodies. Only the region of the gel around the VESA1 complex is shown. The lower panel is a lighter exposure of the same region to facilitate visualization of bands in more heavily exposed samples. Antibodies used include: 4D9, mAb 4D9.1G1; R6, R6a-v1β765; P6, rabbit 6 preimmune serum; MBOC, mAb MBOC79B1. Asterisk and numbering on the left have the same meanings as in Figure 3