A. Human ApoB containing lipoproteins prepared by dextran sulfate precipitation were incubated in 0 or 200 mM glucose without or with test compound as described in Methods. Results were calculated as µg/mg apoB and represent mean ± SEM of 4 experiments under each condition. The amount of glycated apoB with no added glucose was assigned an arbitrary value of 1. a, b, c represent molar ratios of drug to ApoB of 2:1, 4:1 and 8:1, respectively. *p ≤ 0.05 compared to (−) glucose; #p ≤ 0.05 compared to (+) glucose; @p = .05 compared to (+) glucose (+) drug a.
B. Lipid peroxidation in human apoB-containing lipoprotein incubated in 0 or 200 mM glucose without or with test compound as described in Methods. Results calculated as nm TBARs/mg apoB, with 0 glucose assigned an arbitrary value of 1, and represent mean ± SEM of 4 experiments under each condition. *p = 0.05 compared to (−) glucose; #p = 0.05 compared to (+) glucose.