Table 1. MPO deficiency suppresses endotoxemia-induced increases in EpOMEs, DHOMEs, and DHETs.

Plasma levels of EpOMEs (epoxides of LA), DHOMEs (dihydroxy metabolites of LA), and DHETs (dihydroxy metabolites of AA) at 24 h after induction of endotoxemia. Results represent mean ± SEM derived from at least four animals per group. *p < 0.05 MPO-KO vs. wild-type, +p < 0.05 control wild-type vs. LPS treated wild-type, #p < 0.05 control MPO-KO vs. LPS treated MPO-KO. Plasma levels of other determined metabolites including epoxides of AA (EETs) and degradation products 9-oxo-ODE, 13- oxo-ODE, 5-oxo-ETE, and 15-oxo-ETE were under detection limits in both wild-type and MPO-KO mice. Limits of quantification are in Supplement Table 1.

Plasma concentration (nM)	Control WT	Control MPO-KO	LPS WT	LPS MPO-KO
(±)9(10)epoxy-12Z-octadecenoic acid (9(10)-EpOME)	3.5 ± 1.0	4.3 ± 0.7	5.5 ± 1.1	3.0 ± 0.5
(±)12(13)-epoxy-9Z-octadecenoic acid (12(13)-EpOME)	6.0 ± 0.9	6.9 ± 1.1	13.0 ± 2.13 +	7.4 ± 0.8 *
(±)9,10-dihydroxy-12(Z)-octadecenoic acid (9,10-DHOME)	2.7 ± 0.4	4.0 ± 1.0	19.4 ± 4.4 +	11.0 ± 1.4 #
(±)12,13-dihydroxy-9(Z)-octadecenoic acid (12,13-DHOME)	9.1 ± 2.1	12.1 ± 3.8	50.6 ± 9.4 +	30.4 ± 3.1 *#
(±)5,6-dihydroxy-8Z, 11Z, 14Z-eicosatrienoic acid (5,6-DHET)	2.3 ± 0.5	1.6 ± 0.5	6.4 ± 1.7 +	3.0 ± 0.6 *
(±)8,9-dihydroxy-5Z, 11Z, 14Z-eicosatrienoic acid (8,9-DHET)	5.0 ± 1.3	4.9 ± 0.3	12.2 ± 1.2 +	7.7 ± 1.1 *#
(±)11,12-dihydroxy-5Z, 8Z, 14Z-eicosatrienoic acid (11,12-DHET)	3.2 ± 0.5	3.5 ± 0.3	10.6 ± 1.8 +	6.0 ± 0.6 *#
(±)14,15-dihydroxy-5Z,8Z, 11Z-eicosatrienoic acid (14,15-DHET)	5.4 ± 0.7	4.5 ± 0.3	15.8 ± 2.1 +	7.9 ± 0.8 *#