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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Free Radic Biol Med. 2010 Feb 13;48(10):1311–1320. doi: 10.1016/j.freeradbiomed.2010.02.010

Table 2. MPO deficiency suppresses endotoxemia-induced increases in HETEs and HODEs.

Plasma levels of HETEs (AA-derived lipid metabolites) and HODEs (LA-derived lipid metabolites) generated by LOX-catalyzed pathways. HETEs and HODEs were analyzed 24 h after induction of endotoxemia. Results represent mean ± SEM derived from at least four animals per group. *p < 0.05 MPO-KO vs. wild-type, +p < 0.05 control wild-type vs. LPS treated wild-type, #p < 0.05 control MPO-KO vs. LPS treated MPO-KO. Plasma levels of other determined metabolites including 8-HETE, 9-HETE, 19-HETE, and 20-HETE were under detection limits in both wild-type and MPO-KO mice. Limits of quantification are in Supplement Table 1.

Plasma concentration (nM) Control WT Control MPO-KO LPS WT LPS MPO-KO
(±)5-hydroxy-6E, 8Z,11Z, 14Z-eicosatetraenoic acid (5-HETE) 3.1 ± 0.4 2.2 ± 0.2 * 18.4 ± 2.8 + 8.0 ± 1.3 *#
(±)11-hydroxy-5Z, 8Z,12E, 14Z-eicosatetraenoic acid (11-HETE) 1.38 ± 0.34 1.01 ± 0.27 2.39 ± 0.21 + 0.89 ± 0.25 *
(±)12-hydroxy-5E, 8Z,10Z, 14Z-eicosatetraenoic acid (12-HETE) 3485 ± 786 3202 ± 374 4555 ± 665 3869 ± 746 *
(±)12-hydroxy-5E, 8Z,10Z, 14Z-eicosatetraenoic acid (15-HETE) 1.24 ± 0.18 1.19 ± 0.08 2.03 ± 0.31 + 0.97 ± 0.08 *#
(±)9-hydroxy-10E,12Z-octadecadienoic acid (9-HODE) 5.5 ± 1.2 5.2 ± 0.9 10.6 ± 0.7 + 5.8 ± 1.0 *
(±)13-hydroxy-9Z,11E-octadecadienoic acid (13-HODE) 8.4 ± 1.3 7.6 ± 1.3 22.3 ± 2.2 + 11.8 ± 1.2 *#