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. Author manuscript; available in PMC: 2010 Apr 20.
Published in final edited form as: J Neurochem. 2010 Jan 7;112(6):1593–1604. doi: 10.1111/j.1471-4159.2010.06568.x

Fig. 1.

Fig. 1

LRRK2 binds to MKK6, and pathogenic mutations in LRRK2 increase the binding of LRRK2 to MKK6. LRRK2 activates the p38 pathway. (A) LRRK2 co-immunoprecipitates with MKK6 in HEK-293FT cells. (B) LRRK2 kinase and COR domains are essential and sufficient for its interaction with MKK6. (C) Dominant negative K82A mutation in MKK6 reduces MKK6 binding to LRRK2. All experiments have been performed three times. D.) Eluate from anti-V5 column shows the association of endogenous MKK6 with LRRK2 (V5). Levels of each protein in the lysates are shown below.