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. Author manuscript; available in PMC: 2010 Apr 20.
Published in final edited form as: J Neurochem. 2010 Jan 7;112(6):1593–1604. doi: 10.1111/j.1471-4159.2010.06568.x

Fig. 3.

Fig. 3

LRRK2 stimulates MKK phosphorylation. A. Recombinant MKK3, 6 or 7 (Flag-tagged) was incubated ± LRRK2 (WT, G2019S or KD, GST-tagged), in the presence of kinase buffer and 32P-ATP. B. Coomassie gel showing the purity of the recombinant MKK3, 6 and 7. C & D. Quantification of MKK phosphorylation (C) and LRRK2 autophosphorylation (D) by densitometry. Error bars show the SEM from three independent experiments.