Table 1.
Kinetic parameter values (kcat, Km) of incorporation of dATP and dGTP against a template ClU residue with pol β, AMV-RT, and Klenow at pH 7.5.
| pol β | AMV-RT | Klenow | ||
|---|---|---|---|---|
| dATP:ClU | kcat (s−1) | 1.20 × 10−04 ± 1.57 × 10−05 | 3.75 × 10−02 ± 4.81 × 10−03 | 1.06 × 10−03 ± 4.38 × 10−05 |
| Km (M) | 1.65 × 10−07 ± 8.76 × 10−08 | 3.17 × 10−07 ± 1.57 × 10−07 | 6.39 × 10−09 ± 1.44 × 10−09 | |
| dGTP:ClU | kcat (s−1) | 4.95 × 10−05 ± 8.21 × 10−06 | 8.30 × 10−03 ± 1.38 × 10−03 | 2.21 × 10−03 ± 2.05 × 10−04 |
| Km (M) | 2.20 × 10−04 ± 1.31 × 10−04 | 3.90 × 10−04 ± 2.17 × 10−04 | 2.67 × 10−05 ± 9.79 × 10−06 |