Table 2.
Kinetic parameter values (Vmax/Km) of misincorporation of dATP against a template ClU or T residue and incorporation of dGTP against a template ClU and T residue with pol β, AMV-RT, and Klenow determined from three independent data sets.
| pol β | AMV-RT | Klenow | ||
|---|---|---|---|---|
| pH | Vmax/Km(s−1) | |||
| dATP:ClU | 6.5 | 1.18 × 10−05 ± 6.75 × 10−06 | 2.42 × 10−04 ± 2.74 × 10−05 | 8.38 × 10−05 ± 1.42 × 10−05 |
| 7.0 | 2.01 × 10−05 ± 5.99 × 10−06 | 2.83 × 10−04 ± 9.02 × 10−06 | 9.60 × 10−05 ± 1.59 × 10−06 | |
| 7.5 | 3.15 × 10−05 ± 3.55 × 10−06 | 2.80 × 10−04 ± 3.25 × 10−05 | 1.33 × 10−04 ± 1.82 × 10−05 | |
| 8.0 | 5.09 × 10−05 ± 2.63 × 10−06 | 1.97 × 10−04 ± 2.63 × 10−05 | 1.70 × 10−04 ± 1.27 × 10−05 | |
| 8.5 | 5.61 × 10−05 ± 5.78 × 10−06 | 1.03 × 10−05 ± 1.55 × 10−05 | 1.23 × 10−04 ± 2.05 × 10−05 | |
| 9.0 | 4.06 × 10−05 ± 3.92 × 10−06 | 7.59 × 10−05 ± 7.73 × 10−06 | 9.62 × 10−05 ± 1.24 × 10−05 | |
| 9.5 | 3.57 × 10−05 ± 1.04 × 10−06 | N/A | N/A | |
| dATP:T | 6.5 | 1.33 × 10−05 ± 4.72 × 10−06 | 1.82 × 10−04 ± 1.11 × 10−05 | 5.98 × 10−05 ± 4.75 × 10−06 |
| 7.0 | 2.78 × 10−05 ± 4.29 × 10−06 | 2.00 × 10−04 ± 1.05 × 10−05 | 1.29 × 10−04 ± 3.08 × 10−05 | |
| 7.5 | 3.50 × 10−05 ± 8.55 × 10−06 | 2.17 × 10−04 ± 4.17 × 10−06 | 1.76 × 10−04 ± 1.68 × 10−05 | |
| 8.0 | 5.76 × 10−05 ± 1.04 × 10−05 | 1.49 × 10−04 ± 1.46 × 10−05 | 2.14 × 10−04 ± 2.58 × 10−05 | |
| 8.5 | 5.27 × 10−05 ± 1.09 × 10−05 | 8.77 × 10−05 ± 5.83 × 10−06 | 1.53 × 10−04 ± 2.36 × 10−05 | |
| 9.0 | 4.11 × 10−05 ± 1.56 × 10−05 | 5.50 × 10−05 ± 7.48 × 10−06 | 1.05 × 10−04 ± 1.55 × 10−05 | |
| 9.5 | 2.94 × 10−05 ± 1.02 × 10−05 | N/A | N/A | |
| dGTP:ClU | 6.5 | 1.11 × 10−08 ± 4.57 × 10−10 | 1.10 × 10−08 ± 2.07 × 10−09 | 5.93 × 10−08 ± 3.12 × 10−08 |
| 7.0 | 2.01 × 10−08 ± 1.80 × 10−09 | 1.73 × 10−08 ± 2.93 × 10−09 | 7.39 × 10−08 ± 3.85 × 10−09 | |
| 7.5 | 3.56 × 10−08 ± 4.87 × 10−09 | 4.72 × 10−08 ± 1.34 × 10−09 | 1.21 × 10−07 ± 2.32 × 10−08 | |
| 8.0 | 1.01 × 10−07 ± 1.16 × 10−08 | 8.60 × 10−08 ± 3.89 × 10−09 | 2.86 × 10−07 ± 2.69 × 10−08 | |
| 8.5 | 3.21 × 10−07 ± 1.69 × 10−08 | 1.04 × 10−07 ± 1.49 × 10−09 | 3.90 × 10−07 ± 2.68 × 10−08 | |
| 9.0 | 4.65 × 10−07 ± 1.12 × 10−08 | 1.08 × 10−07 ± 4.90 × 10−09 | 4.61 × 10−07 ± 4.45 × 10−08 | |
| 9.5 | 5.56 × 10−07 ± 1.56 × 10−08 | N/A | N/A | |
| dGTP:T | 6.5 | 4.91 × 10−09 ± 1.06 × 10−09 | 1.33 × 10−08 ± 1.21 × 10−09 | 2.64 × 10−08 ± 1.65 × 10−08 |
| 7.0 | 7.84 × 10−09 ± 9.51 × 10−10 | 1.72 × 10−08 ± 1.14 × 10−09 | 4.72 × 10−08 ± 1.42 × 10−09 | |
| 7.5 | 9.70 × 10−09 ± 2.23 × 10−09 | 2.79 × 10−08 ± 4.82 × 10−09 | 8.00 × 10−08 ± 1.41 × 10−08 | |
| 8.0 | 1.52 × 10−08 ± 2.62 × 10−09 | 4.48 × 10−08 ± 2.77 × 10−09 | 1.77 × 10−07 ± 1.10 × 10−08 | |
| 8.5 | 1.77 × 10−08 ± 1.81 × 10−09 | 5.91 × 10−08 ± 2.47 × 10−09 | 2.22 × 10−07 ± 1.43 × 10−08 | |
| 9.0 | 5.20 × 10−08 ± 6.47 × 10−09 | 2.06 × 10−08 ± 6.23 × 10−09 | 2.59 × 10−07 ± 9.27 × 10−09 | |
| 9.5 | 1.08 × 10−07 ± 1.20 × 10−08 | N/A | N/A | |