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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Thromb Haemost. 2009 Dec 11;8(3):548–555. doi: 10.1111/j.1538-7836.2009.03723.x

Fig. 1.

Fig. 1

PolyP binds to thrombin but not prothrombin. (A) Gel-shift mobility assay in which 1 µg thrombin (T) or prothrombin (PT) were incubated ± 100 µg polyP25, resolved on native PAGE and stained with toluidine blue to detect polyP. Free polyP25 migrated at the dye front. (B) Thrombin (27 pmol) or prothrombin (143 pmol) were incubated with polyP-zirconia after which fractions were resolved on SDS-PAGE and probed with anti-prothrombin antibody. Lanes: 1, starting material; 2, flow-through; and 3, high salt (1 M NaCl) eluate. (C) Zirconia beads with (+) or without (−) attached polyP75 were incubated with biotin-thrombin in plasma or buffer. Bound thrombin was eluted with high salt buffer, resolved on SDS-PAGE and probed with avidin-peroxidase (representative of four blots).