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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Thromb Haemost. 2009 Dec 11;8(3):548–555. doi: 10.1111/j.1538-7836.2009.03723.x

Fig. 3.

Fig. 3

Exosite I is not involved in polyP binding to thrombin. (A) α-thrombin (●) or γ-thrombin (▲), both at 27 pmol were incubated with polyP-zirconia beads in binding buffer with varying NaCl concentrations, after which the flow-through was analyzed for thrombin activity. (B) Thrombin (27 pmol) was preincubated for 30 min with vehicle alone (open bars) or with the C-terminal dodecapeptide of hirudin (solid bars; 5 µg/ml), after which the mixtures were incubated with polyP-zirconia beads in binding buffer containing 50 mM or 1 M NaCl. Thrombin activity was quantified in the flow-through. Data represent mean ± S.D. (n = 3).