Table 1.
Kd (nM) at 50 mM NaCl* | Kd (nM) at 150 mM NaCl* | |
---|---|---|
WT thrombin | 4.9 ± 0.5 | 5.4 ± 0.4 |
K248A {236} mutant | 12.2 ± 1.6 | NB† |
Binding of polyP75 to immobilized biotin-thrombin (WT and mutant) was quantified in surface plasmon resonance studies at 50 mM and 150 mM NaCl. Kd values in this table are given in terms of polyP polymer concentration (mean ± S.D.; n = 3).
NB, negligible binding.