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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Neurochem. 2009 Nov 6;112(6):1431–1441. doi: 10.1111/j.1471-4159.2009.06479.x

Fig. 5. μ and κ opioid agonists stmulate ESC-derived oligodendrogenesis via their receptors.

Fig. 5

Glial progenitors were treated with DAMGO (1 μM) or U69,593 (1 μM) for 9 days. In some experiments, 1 μM CTAP or 1 μM nor-BNI were added to cells 1 h prior and during opioid agonist treatment. Fresh agonists and antagonists were added on a daily basis. Then cells were fixed, permeabilized and labeled with a mouse monoclonal anti-O4 Ab (5 μg/mL), followed by labeling with a mouse IgM Alexa Fluor 594 (1: 1000) + DAPI (1:250). (A) Representative images of O4+ (red) and DAPI+ (blue) cells. (B) O4+ cell counting data are expressed as % controls (%oligodendrocytes vs. total number of DAPI+ cells). N = 3. *P<0.05, ***p< 0.001 vs. controls; #P<0.05 vs. corresponding agonist alone.