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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Int J Cancer. 2010 Jun 15;126(12):2893–2903. doi: 10.1002/ijc.24995

Figure 2. Polyclonal anti-HER2 Vaccine Induced Antibodies (Ad-HER2-VIA) lead to HER2 receptor internalization and degradation.

Figure 2

(a) Western blots of ErbB2, in SKBR3 cells at 72 hours after designated treatments. (1 = no treatment, 2 = lapatinib 0.2 micro-Molar, 3 = HER2-VIA (1:50), 4 = LacZ-VIA (1:50). (b) HER2-GFP transfected HEK293 cells were plated onto collagen-coated plastic dishes with glass bottoms and cultured overnight. Confocal images of cells expressing HER 2-GFP were acquired using Zeiss laser scanning microscopy (LSM-510). Cells were left untreated (top row) or treated for 1h with HER2-vaccine induced antibodies (HER2-VIA, bottom left), LacZ-vaccine induced antibodies (LacZ-VIA, bottom middle), or trastuzumab (bottom right). Representative images of three independent experiments are shown.