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Apoptotic cells with PPP or LY294002. Under the microscope, cells with pyknotic nuclei (arrows) were observed in medium with PPP or LY294002, whereas there were no apoptotic cells in medium with PD98059. FBS: Medium with 100 mL/L FBS; PPP: Medium with 2 μmol/L PPP; LY: Medium with 50 μmol/L LY294002; and PD: Medium with 20 μmol/L PD98059. Original magnification: 20 ×; Scale bar: 25 μm, the arrows indicate cells in apoptosis.
