FIGURE 3.

miR-382 levels are primarily responsive to the exosome complex. (A) HEK293T cells were transduced with lentiviruses expressing shRNAs directed either to Xrn1, Xrn2, or Rrp41. Levels of the indicated mRNAs in control and shRNA-expressing cells as determined by quantitative real time–PCR are graphed. Xrn1, Xrn2, and Rrp41 mRNA levels were reduced by 85%, 83%, and 78% in the respective knockdown cells. (B) miR-382 and miR-378 levels were determined using the TaqMan miRNA assay kit (Applied Biosystems) in each indicated knockdown cell line and presented relative to RNU43 snoRNA. Data represent the mean of at least three independent experiments ± standard errors.