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. 2010 May;16(5):1062–1067. doi: 10.1261/rna.2049510

FIGURE 3.

FIGURE 3.

(−) Strand Y-Sat siRNAs are detected at similar levels as (+) strand siRNAs in the presence of in vitro antisense Y-Sat transcript. (A) Detection of (−) strand Y-Sat siRNAs in the presence (lanes 2–5) or absence (lanes 6,7) of in vitro antisense Y-Sat transcript. (B) Detection of (+) strand Y-Sat siRNAs in the presence (lanes 2,3) or absence (lane 1) of an in vitro–transcribed sense Y-Sat transcript. Blotted membranes were hybridized with an oligonucleotide probe containing a 31-nt U6 RNA sequence fused to either a 40-nt sense (A) or antisense (B) Y-Sat sequence (Supplemental Data 1). (Bottom) Shorter exposure of the U6 RNA signal in the top panels to indicate even loading. Ten micrograms of total RNA were loaded for each sample. The gray arrows indicate the position of the 21-nt siRNA species.