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. 2010 Apr 19;189(2):223–232. doi: 10.1083/jcb.200911018

Figure 2.

Figure 2.

Identification of Rab35 as the target of TBC1D10A–C. Biochemical GAP assays were performed using recombinant hexahistidine-GST–tagged human Rab GTPases and hexahistidine-tagged TBC1D10 family proteins. Reactions were performed for 60 min at 37°C, using 100 pmol GST-Rab and 10 pmol hexahistidine-tagged TBC1D10A, -B, or -C. GTP hydrolysis is plotted in picomoles per hour. Error bars represent SD.