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. 2010 Apr 19;189(2):223–232. doi: 10.1083/jcb.200911018

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© 2010 Hsu et al.

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Figure 3. — Inhibition of Rab35 function reduces exosome secretion of PLP. (A) Cells were transfected with PLP-myc, TBC1D10B, and either YFP or Rab35^Q67A. The amounts of PLP in the cell lysates (CL) and 100,000 g pellets (P100) were quantified, and the results of two experiments performed in duplicates are shown in B (values represent the mean ± SD; *, P < 0.05; Welch’s two-sample t test). (C) Cells were transfected with PLP-myc together with either wild-type (WT) Rab35 or the dominant-negative mutant (Rab35^N120I). (D and E) After delivery of control (Ctl) or siRNA against Rab35 into cells by nucleofection, the cell lysates were analyzed by Western blotting with Rab35 antibodies to monitor knockdown efficiency. Actin and Rab5 were used as controls. The amount of PLP in the cell lysates and 100,000 g pellets were determined after Rab35 RNAi. (F) Results are expressed as the mean ± SD of four experiments (*, P < 0.05; one-sample t test and Welch’s two-sample t test).