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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Eye Res. 2010 Feb 10;90(5):572–582. doi: 10.1016/j.exer.2010.02.001

Figure 7. Pax6 is required for cone, but not rod, cell regeneration.

Figure 7

Dark-adapted adult albino zebrafish were either uninjected (A, F, K) or injected and electroporated with the Standard Control morpholino (B, G, L), with anti-pax6a morpholino (C, H, M), anti-pax6b morpholino (D, I, N), or a cocktail of both morpholinos (E, J, O) and exposed to constant intense light for 96 hours. Retinas were examined 28 days following the completion of the constant light treatment. Rhodopsin immunolocalization (A–E, red) suggested that equivalent numbers of rod photoreceptors regenerated in the uninjected and morphant retinas. The pax6b morphant and pax6a/pax6b double morphant retinas contained significantly fewer long and short single cones relative to the controls, as visualized by blue opsin (F–J, blue) and UV opsin (K–O, green) immunolocalization, respectively. In contrast, the pax6a morphant retinas possessed an intermediate number of long and short single cones relative to the controls and the pax6b morphants. The scale bar in panel A represents 25 microns and is the same for panels B–O.