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. Author manuscript; available in PMC: 2010 Apr 20.
Published in final edited form as: J Phycol. 2006 Dec 1;42(6):1333–1348. doi: 10.1111/j.1529-8817.2006.00285.x

Fig. 4.

Fig. 4

Serial dilution of plasmid containing the 28S rRNA target for the H. akashiwo real-time PCR assay. The plasmid was prepared by ligating the target amplicon into pCR® 2.1 vector and transforming into One Shot® Top 10F′ chemically competent cells. The lower limit of the assay was 7.40 copies of the target in the reaction.