Table 2.
Primers used to generate and sequence overlapping reads for target loci (18S; ITS1-5.8S-ITS2; 16S).
| Sequence 5′–3′ | Specificity | |
|---|---|---|
| 18S primersa | ||
| ChattR | GCTGACGGAGTCGTTGAG | Group (Raphidophyte) specific |
| 4617F | TCCTGCCAGTAGTCATATGC | Eukaryotic general |
| 35F | GTCTCAAAGATTAAGCCATGC | Eukaryotic general |
| 128F/R | TACTGTGAAACTGCGAATGGCTC | Eukaryotic general |
| 516F/R | CACATCTAAGGAAGGCAGCA | Eukaryotic general |
| 683F/R | TTGGAGGGCAAGTCTGGTGCC | Eukaryotic general |
| 1072F | GGGATAGTTGGGGGTATTCGTATT | Eukaryotic general |
| 1416R | TTCAGCCTTGCGACCATACTC | Eukaryotic general |
| 1590F | AACGAGACCCCCGCCTGCTAA | Eukaryotic general |
| 1815F/R | GGAAGTTGGGGGCAATAACAGG | Eukaryotic general |
| 4618Rb | TGATCCTTCTGCAGGTTCACCTAC | Eukaryotic general |
| ITS1-5.8S-ITS2 primersa | ||
| 4618F | GTAGGTGAACCTGCAGAAGGATCA | Eukaryotic general |
| ITSF/R | ATGTCTTGGTTCCCACAACGATG | Semi-eukaryotic specific |
| LSU1R | ATATGCTTAAATTCAGCGGGT | Eukaryotic general |
| 16S primersa,c | ||
| 880F/R | ATAACTGACACTCAGAGACGACA | Plastid specific |
| 1809R | CAGTACGGCTACCTTGTTACGA | Prokaryotic general |
a
F (forward) and R (reverse) correspond to the direction of the primer.
c
Additional primers were utilized to generate the plastid sequence for F. japonica CCMP 1661 (Tengs et al. 2000).