FIGURE 3.

Aae MutL (E357K) and Aae MutL-CTD stimulate Aq793 helicase activity. Aq793 was assayed for unwinding activity in the presence of serial dilutions of Aae MutL (E357K) and Aae MutL-CTD. The reactions were performed as described in the legend to Fig. 2 and under “Materials and Methods.” A, representative TBE acrylamide gel of the titration of Aae MutL-CTD (top gel picture) and Aae MutL (E357K) (bottom gel picture) mutants. Negative controls (−) contained Aq793 protein but not the respective MutL mutant. B, graphical representation of the effect of Aae MutL-CTD (solid squares) and Aae MutL (E357K) (open circles) on the oligonucleotide displacement activity of Aq793. Gel bands were quantified using ImageQuant TL software, and individual points represent the averages of three separate experiments.