FIGURE 2.

The sulfotransferase activity of 2OST WT and Y94A mutant toward different polysaccharides. Different amounts of 2OST proteins (0–1.0 μg) were incubated with either 2.0 μg of N-sulfoheparosan (A) or 2.0 μg of CDSNS heparin (B) and 5.9 × 10⁵ cpm of [³⁵S]PAPS for 5 min at 37 °C. The sulfated polysaccharide was purified by DEAE resin and was determined by scintillation counting. The results for 2OST WT are shown by filled circles, and results for 2OST Y94A are shown in empty circles.