FIGURE 3.

The expression and the sulfotransferase activity of 2OST mutants. The 2OST mutants were overexpressed in Origami-B cells using pMalc2x vector. The purified proteins were analyzed by 10% SDS-PAGE with 2OST WT (A). The sulfotransferase activities of these mutants were tested using either N-sulfoheparosan or CDSNS heparin as a substrate and are shown in percentage relative to wild type enzyme (B). The results are presented as average ± S.D., which was calculated based on three determinations.