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Activities of different truncations of C5-epi. The different truncations of human heparan sulfate C5 epimerase were overexpressed in Origami-B cells using the pMalc2x vector. A, purified proteins analyzed by SDS-PAGE. The values (average ± S.D.) for the activity measured by the developed assay (5.9 × 105 cpm of [35S]PAPS) are shown in B. The bars indicate S.E. of triplicates.
