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Analysis of tyrosine mutants of C5-epi. The tyrosine mutants were prepared by site-directed mutagenesis using the plasmid containing a truncated form of human C5-epi (Glu53–Asn617) as template. The purified proteins were analyzed by SDS-PAGE, shown in A. The activities (average ± range) are shown in B. The bars indicate the range of assay results for epimerase mutants prepared from two different batches.
