TABLE 1.

Summary of disaccharide analysis for C₅-epi- and 2OST-modified HS

C5-epi mutants	IdoUA2S-AnMan/GlcUA2S-AnMan	Relative C5-epi activitya
		%
No C5-epi	≤0.1
WT	5.3	100
Y146A	2.2	40
Y162A	2.2	23
Y168A	≤0.1	Inactive
Y210A	6.2	71
Y222A	≤0.1	Inactive

^a The relative activities were calculated from the data shown in Fig. 8.