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Effects of PGIS overexpression in insulin-producing RINm5F cells on cytokine-stimulated iNOS protein expression. The cells were incubated with IL-1β (600 units/ml) or a cytokine mixture (60 units/ml IL-1β, 185 units/ml TNFα, and 14 units/ml IFNγ) for 24 h, and thereafter the samples were collected for Western-blotting. A representative blot from four independent experiments is shown. con, control. Mix, cytokine mixture.
