. 2010 Feb 16;285(15):11121–11128. doi: 10.1074/jbc.M109.054775

TABLE 2.

Effects of PGIS overexpression in RINm5F insulin-producing cells on cell proliferation after exposure to IL-1β alone or a cytokine mixture

RINm5F insulin-producing cells overexpressing PGIS as well as control cells were incubated with IL-1β (600 units/ml) alone or a cytokine mixture (60 units/ml IL-1β, 185 units/ml TNF-α, and 14 units/ml IFNγ) for 72 h. The proliferation rate of the cells was determined by the bromodeoxyuridine-enzyme-linked immunosorbent assay and expressed as a percentage of the untreated cells. The data are the means ± S.E. with the numbers of experiments in parentheses, each measured in at least three repetitions (analysis of variance followed by Bonferroni).

RINm5F cell clone	Cell proliferation
RINm5F cell clone	Untreated	IL-1β	Mix
RINm5F	100 ± 3 (4)	88 ± 3 (4)	55 ± 3 (4)^a
RIN-PGIS K1	100 ± 5 (4)	91 ± 6 (4)	73 ± 6 (4)^a
RIN-PGIS K2	100 ± 4 (4)	93 ± 3 (4)	75 ± 4 (4)^a
RIN-PGIS K3	100 ± 4 (4)	97 ± 4 (4)	80 ± 4 (4)^a^,^b
RIN-PGIS K4	100 ± 1 (4)	102 ± 4 (4)	84 ± 5 (4)^b

^a p < 0.05 versus untreated.

^b p < 0.05 cytokine mixture versus control clone.