TABLE 4.

Effects of PGIS overexpression in RINm5F insulin-producing cells on the glucose oxidation rate after exposure to IL-1β alone or a cytokine mix

RINm5F insulin-producing cells overexpressing PGIS as well as control cells were incubated with IL-1β (600 units/ml) alone or a cytokine mixture (60 units/ml IL-1β, 185 units/ml TNF-α, and 14 units/ml IFNγ) for 72 h. Glucose oxidation rates at 10 mmol/liter [U-¹⁴C]glucose (1Ci/mol) were expressed as a percentage of untreated cells. Glucose oxidation rates under control conditions were identical in the control cells (0.09 ± 0.005 mmol/g of DNA/h) and in the RIN-PGIS K4 clone (0.12 ± 0.003 mmol/g of DNA/h). The data are the means ± S.E. with the numbers of experiments in parentheses, each measured in two repetitions (analysis of variance followed by Bonferroni).

RINm5F cell clone	Glucose oxidation rate
	Untreated	IL-1β	Cytokine mixture
RINm5F	100 ± 3 (8)	63 ± 19 (7)a	34 ± 7 (8)a
RIN-PGIS K4	100 ± 1 (8)	286 ± 57 (6)a,b	376 ± 41 (7)a,c

^a p < 0.05 versus untreated.

^b p < 0.05 IL-1β versus control clone.

^c p < 0.05 cytokine mixture versus control clone.