FIGURE 5.

Physical interactions of Pitx2 and cofactors. A, nuclear extracts prepared from the abdominal wall tissue of E10.5 WT tissue were immunoprecipitated with antibodies (Ab) against Pitx2, HDAC1, HDAC3, and N-CoR and hybridized with the same antibodies. 10% input and immunoprecipitates without specific antibodies were used as controls. Pitx2 interacted with HDAC1, HDAC3, and N-CoR. HDAC1 interacted with Pitx2 and N-CoR. HDAC3 interacted with Pitx2 and N-CoR. N-CoR interacted with Pitx2, HDAC1, and HDAC3. B, diagrammatic representation of the Pitx2 cofactorial complexes. Pitx2 was in a complex with N-CoR and HDAC1 and in a different complex with N-CoR and HDAC3.