TABLE 2.

Pitx2 occupancy at T-box genes

Gene	Amplicon number	Amplicon location	% of IP WT	% of IP MUT	% of IP no Aba
Tbx1	0	none (−3800, +10,013)b	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	1	−9409	19.1 ± 1.3	<0.1 ± 0.01	<0.1 ± 0.01
	2	−2711	26.3 ± 3.1	<0.1 ± 0.01	<0.1 ± 0.01
	3	−1335	18.3 ± 1.6	<0.1 ± 0.01	<0.1 ± 0.01
	4	+1691	7.2 ± 3.0	<0.1 ± 0.01	<0.1 ± 0.01
	5	+7153	3.0 ± 0.5	0.2 ± 0.04	<0.1 ± 0.01
Tbx15	1	−7619, −7568	11.9 ± 0.3	<0.1 ± 0.01	<0.1 ± 0.01
Tbx2	1	−18,063	0.9 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	2	+4279, +4500	32.8 ± 0.9	0.8 ± 0.1	0.4 ± 0.2
	3	+9149	1.1 ± 0.2	0.1 ± 0.01	0.1 ± 0.01
Tbx4	0	none (+422)a	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	1	−48,363	1.1 ± 0.2	0.1 ± 0.01	0.1 ± 0.01
	2	−17,798, −17,671	1.40 ± 0.1	<0.1 ± 0.01	<0.1 ± 0.01
	3	−15,585, −15,510, −15,482	2.6 ± 0.6	<0.1 ± 0.01	<0.1 ± 0.01
	4	−13,589, −13,401	2.1 ± 0.3	<0.1 ± 0.01	<0.1 ± 0.01
	5	−13,142, −13,079	0.7 ± 0.3	<0.1 ± 0.01	<0.1 ± 0.01
	6	−10,654	0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	7	−9923, −9849	17.1 ± 4.9	0.3 ± 0.1	0.1 ± 0.1
	8	−8167	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	9	−7289	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	10	−3945	25.8 ± 3.8	<0.1 ± 0.01	<0.1 ± 0.01
	11	−3286	7.7 ± 0.2	0.1 ± 0.01	<0.1 ± 0.01
	12	−2000	7.4 ± 0.7	0.1 ± 0.01	0.02 ± 0.01
	13	+3288	10.5 ± 1.2	<0.1 ± 0.01	<0.1 ± 0.01
	14	+3932	7.3 ± 1.3	<0.1 ± 0.01	<0.1 ± 0.01
	15	+8505	1.8 ± 0.3	0.2 ± 0.05	0.1 ± 0.01
	16	+11,161	2.4 ± 0.5	<0.1 ± 0.01	<0.1 ± 0.01
	17	+13,839, +13,865	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
Tbx5	1	−1424	30.7 ± 2.2	0.5 ± 0.2	0.2 ± 0.1
	2	+4347	0.6 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
Tbx6	1	−4917	10.6 ± 1.1	0.2 ± 0.05	0.1 ± 0.01
	2	−2542, −2437	12.4 ± 0.4	0.4 ± 0.1	0.4 ± 0.1
	3	−1728	17.5 ± 3.4	0.5 ± 0.1	0.4 ± 0.2
Eomes	1	−1579	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	2	+8879	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	3	+9518	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
Mga	1	−3464, −3399, −3297	<0.1 ± 0.01	<0.1 ± 0.01	<0.1 ± 0.01
	2	+4251, +4270, +4279	7.6 ± 0.9	0.1 ± 0.01	<0.1 ± 0.01
		+4353, +4418, +4423

^a no Ab, no-antibody control.

^b Sites without Pitx2 binding motifs.