FIGURE 3.

porin hypomorphic mutant homozygotes are infertile/subfertile. A, male fertility assay. Bar graph shows mean number of progeny observed from mating individual males of the indicated genotypes with yw virgin females. c135-GAL4 is a GAL4 driver that is expressed throughout the male reproductive tract (23). Error bars represent S.E. Sample sizes: yw (n = 21), porin^EY2333 (n = 51), porin^Rev8 (n = 81), rescued porin^Rev8 (n = 74). Western blot of testis lysates from males of the indicated genotypes is also shown. B, a representative dissected male reproductive tract is shown for yw (right) and porin^Rev8 (left). A bar graph indicating the mean greatest width of testes for both genotypes is shown below the picture. Error bars represent S.E. Sample sizes: yw (n = 15), porin^Rev8 (n = 14). C, Female fecundity/fertility assays. Bar graphs show mean number of eggs laid (top), mean number of adult progeny (middle), and mean ratio of progeny to eggs laid (bottom) observed for females of the indicated genotypes mated with yw males. Statistical significance evaluated by Student's t test with Welch correction (compared with yw). Error bars represent S.E. Sample sizes: yw (n = 10), porin^EY2333 (n = 9), and porin^Rev8 (n = 9). EY2333, porin^EY2333; Rev8, porin^Rev8.